Johan Sebastiaan Ploem

He was invited for a symposium on ‘Art et Science’ at the University of Caen, France (April, 2001). At the art exposition connected with this symposium, he presented 6 digital graphics that were dominated by chaotic transformations of rock art themes. A similar invitation was made by the University of Basel in Switzerland (April, 2002).

At the International Symposium on Mathematical Morphology in Amsterdam (1998), J.S Ploem presented a paper on the creation of computer graphics with Mathematical Morphology, using for the first time, the transforming algorithms from the Fontainebleau group for the creation of digital art. (Mathematical Morphology and its Applications to Image and Signal Processing, Heijmans and Roerdink (Eds), Kluwer. 1098: page 355:) Ploem described and developed methodology for the creation of transformational digital art.

Ploem was probably the first person to systematically use mathematical morphology for the creation of digital art, Ploem's work attracted international attention and he was invited as a plenary speaker at an international mathematical conference in Amsterdam in 1998 to explain his new type of digital art. He also received an invitation to show his art work in an exposition at this conference. The organisers of this meeting asked Ploem to write a chapter on his novel technique for digital art in a book (Kluwer, ISBN 0-7923-5133-9) that was published on the occasion of this meeting.

His first digital graphics of nature scenes were shown in his exposition at a regional art centre in the Pyrenees (Ossega, June, 1997).

In 1973, at the Second Conference on Mononuclear Phagocytes held in Leiden, Ploem introduced an improvement to Interference Reflection Microscopy (IRM), which he called Reflection Contrast Microscopy (RCM). He also wrote a book chapter in the associated conference proceeding edited by Ralph van Furth. The improvement is the addition of crossed polarizers and a so-called "anti-flex objective", the combination of which further reduces stray light in an IRM microscope, allowing even better interference contrast. RCM is more commonly known as Reflection Contrast Interference Microscopy (RICM) today.

Ploem is Professor Emeritus at Leiden University, the Netherlands. He is a graduate of Utrecht University, the Netherlands, receiving an MD in 1972. He worked as Intern in the Broussais Hospital (Paris, France) with Professor Pasteur Valery-Radot. In 1963, Dr. Ploem was elected a Fulbright Fellow for Study at the Harvard University School of Public Health, receiving a Master of Public Health degree Cum Laude in 1954. He obtained a Ph.D. degree in 1967 from the University of Amsterdam, the Netherlands. Professor Ploem has served as visiting lecturer or professor at various universities: Dundee. Scotland; University of Florida, USA; Monash University, Melbourne, Australia; University of Beijing, China; and at the Free University of Brussels, Belgium. In 1980, he was appointed to a professorship in the Department of Cytochemistry and Cytometry at Leiden University. He retired from that position in 1992. Numerous honors and awards have been bestowed on Professor Ploem. In 1976 he was elected to the honorary Fellowship of the Royal Microscopical Society, Oxford England. In 1977 he received a Fellowship to the Papanicolaou Cancer Research Institute in Miami, Florida, and in 1979 a Fellowship to the Institute for Cell Analysis at the University of Miami, Florida. In 1982, he was the co-recipient of the C. E. Alken Foundation Award, Switzerland. In 1993, he was elected as the first Honorary Member of the International Society for Analytical Cytology. In 1993, Professor Ploem held the Erica Wachtel Medal Lecture at the British Society for Clinical Cytology meeting. In 1994, the European Society for Analytical Cellular Pathology established a Conference Keynote “Ploem” Lecture for invited scientists at its future general meetings. The International Society of Analytical Cytology invited Professor Ploem to present its inaugural “Robert Hooke” lecture. In 1995, he was invited by the Royal Microscopical Society to give the inaugural CYTO lecture.

Around 1962 Ploem started work in collaboration with Schott on the development of dichroic beam splitters for reflection of blue and green light for fluorescence microscopy using epi illumination. At the time of his first publication on fluorescence microscopy using epi illumination with narrow-band blue and green light, he was not aware of the development of a dichroic beam splitter for UV excitation with incident light by Brumberg and Krylova.

Johan Sebastiaan Ploem HonFRMS (born 25 August 1927, Sawahlunto) is a Dutch microscopist and digital artist, who made significant contribution to the field of fluorescence microscopy.